Journal: Cancer letters

Article Title: CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.

doi: 10.1016/j.canlet.2019.10.010

Figure Lengend Snippet: Fig. 1. Analysis of CD44 gene expression in the TCGA data base for HNSCCs using the UCSC Xena Browser. (A) Comparison of CD44 gene expression in normal solid tissue and in primary HNSCC tumors. (B) Comparison of CD44 gene expression in HPV(+) and HPV(−) HNSCC patients. (C) Heat-map from the UCSC Xena Browser based on the primary TCGA HNSCC cohort depicted the gene expression relationship between CD44 and HAS1 (hyaluronan synthase 1), HAS2 (hyaluronan synthase 2) and HAS3 (hyaluronan synthase 3) in 566 patients. (D) Correlation of CD44 gene expression with angiogenesis-related genes in TCGA database (n = 566). (E) Comparison of correlation of CD44 gene expression with angiogenesis-related genes in HPV(+) (n = 39) and HPV(−) (n = 75) HNSCC patients.

Article Snippet: For immunofluorescence staining, sections were incubated with a rabbit anti-mouse CD31 Ab (1:60, Proteintech, Manchester, UK) or a rabbit anti-mouse CD44 Ab (1:100, Acris Antibodies GmbH, Herford, Germany) overnight at 4 °C followed by incubation with the secondary Alexa Fluor® 488 AffiniPure antibody (1:400, Jackson Immuno Research Laboratories, Inc., West Grove, PA, USA).

Techniques: Gene Expression, Comparison

Journal: Cancer letters

Article Title: CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.

doi: 10.1016/j.canlet.2019.10.010

Figure Lengend Snippet: Fig. 2. CD44 expression in HNSCC assessed by immunohistochemistry on tissue microarrays. (A) Representative immunohistochemical staining of CD34, CD105 and CD44 in specimen of control oral mucosa, dysplastic mucosa and HNSCC ( × 200). For HNSCC patients one representative image with low expression and one representative image with high expression for each marker is shown. (B) Correlation of H index of CD44 expression with MVD/CD34 or MVD/CD105 in control oral mucosa, dysplastic mucosa and HNSCC.

Article Snippet: For immunofluorescence staining, sections were incubated with a rabbit anti-mouse CD31 Ab (1:60, Proteintech, Manchester, UK) or a rabbit anti-mouse CD44 Ab (1:100, Acris Antibodies GmbH, Herford, Germany) overnight at 4 °C followed by incubation with the secondary Alexa Fluor® 488 AffiniPure antibody (1:400, Jackson Immuno Research Laboratories, Inc., West Grove, PA, USA).

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Staining, Control, Marker

Journal: Cancer letters

Article Title: CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.

doi: 10.1016/j.canlet.2019.10.010

Figure Lengend Snippet: Fig. 3. CD44 expression during carcinogenesis in 4-NQO-treated mice. (A) Representative images of sections of 4-NQO oral carcinogenesis. The images show immunofluorescence staining for CD44 (red fluorescence) and CD31 (green fluorescence) with DAPI counterstaining (blue fluorescence) at 10x or 20x magnification as indicated. (B) Equivalent staining on human HNSCC tissue. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For immunofluorescence staining, sections were incubated with a rabbit anti-mouse CD31 Ab (1:60, Proteintech, Manchester, UK) or a rabbit anti-mouse CD44 Ab (1:100, Acris Antibodies GmbH, Herford, Germany) overnight at 4 °C followed by incubation with the secondary Alexa Fluor® 488 AffiniPure antibody (1:400, Jackson Immuno Research Laboratories, Inc., West Grove, PA, USA).

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence

Journal: Cancer letters

Article Title: CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.

doi: 10.1016/j.canlet.2019.10.010

Figure Lengend Snippet: Fig. 5. Angiogenic response to implanted CD44(+) cells in dorsal skinfold chamber model. (A) Representative IVM images taken at 10 days after implantation. (B) Microvascular density (MVD) of newly formed microvessels, in regions of interest, expressed in units of cm/cm2 immediately (day 0) and 3, 6, and 10 days after implantation of the gelatin sponges into dorsal skinfold chambers in NOD/SCID mice. (C) Macromolecular leakage as an indicator of microvascular permeability expressed as percent increase compared to previous measurement. (D) Quantitative analysis of CD44(+) and (E) CD31(+) signals by immunofluorescent staining. Data in D and E are expressed as the percentage of the area that was positively stained from the region of interest (% ROI). (F) Representative histological stainings of the dorsal skinfold chamber 10 days after implantation of gelatin sponges in 20x magnification. HE-Staining (1: Matrigel®/gelatin sponge. 2: musculus panniculus carnosus. 3: subcutis). Immunofluorescence staining for the detection of CD44(+) cells or CD31(+) cells (green fluorescence). White arrows: Endothelial structures. Sections were counterstained with DAPI (blue fluorescence). All values represent means ± SEM; *p < 0.05 vs. the control group on the same day; #p < 0.001 vs. any other group on the same day; °p < 0.05 vs. the same group on the previous day. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For immunofluorescence staining, sections were incubated with a rabbit anti-mouse CD31 Ab (1:60, Proteintech, Manchester, UK) or a rabbit anti-mouse CD44 Ab (1:100, Acris Antibodies GmbH, Herford, Germany) overnight at 4 °C followed by incubation with the secondary Alexa Fluor® 488 AffiniPure antibody (1:400, Jackson Immuno Research Laboratories, Inc., West Grove, PA, USA).

Techniques: Permeability, Staining, Immunofluorescence, Fluorescence, Control

Journal: Cancer letters

Article Title: CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.

doi: 10.1016/j.canlet.2019.10.010

Figure Lengend Snippet: Fig. 6. The angiogenesis antibody arrays (A) show the analysis of 200 μg total protein of CD44(+) cell lysate (left array) and CD44(−) cell lysate (right array) regarding their molecular content. The arrays were quantified in (B) using ImageJ. Values are normalized to reference spots on the membranes (top left, top right, and bottom left). (C) CD44 expression by TZ291013b, PCI-30, UMSCC47 and UMSCC90 cells (left to right). (D) Multicolor flow cytometry for CD44 and LAP. Histograms show LAP expression within the CD44(+) or CD44(−) population for TZ291013b, PCI-30, UMSCC47 and UMSCC90 cells (left to right).

Article Snippet: For immunofluorescence staining, sections were incubated with a rabbit anti-mouse CD31 Ab (1:60, Proteintech, Manchester, UK) or a rabbit anti-mouse CD44 Ab (1:100, Acris Antibodies GmbH, Herford, Germany) overnight at 4 °C followed by incubation with the secondary Alexa Fluor® 488 AffiniPure antibody (1:400, Jackson Immuno Research Laboratories, Inc., West Grove, PA, USA).

Techniques: Expressing, Flow Cytometry

Journal: Oncotarget

Article Title: A patient-derived orthotopic xenograft model enabling human high-grade urothelial cell carcinoma of the bladder tumor implantation, growth, angiogenesis, and metastasis

doi: 10.18632/oncotarget.26024

Figure Lengend Snippet: Primary UCC patient bladder tumor specimens (BlCaPt15 and BlCaPt37), and bladder/tumor from their corresponding xenografts and UM-UC-3 cell xenografts in the IB model (same experiments as in Figure ) were sectioned and stained by H&E or IHC with antibodies against mouse and human αSMA, human Ki67, or human CD44. H&E stain shows tumor nests dissecting into smooth muscle bundles. SMA stain highlights these muscle bundles and shows tumor cells dissecting through them. Photographs were taken using a digital deconvoluting microscope and Slide Book 6.0 software. The brown color indicates positive staining. Original magnification 200×.

Article Snippet: Rabbit antibodies (Abs) against human Ki67 (Ki67, Thermo Fisher Scientific, Inc, Waltham, MA), human CD44 (CD44, Acris Antibodies, Inc, Rockville, MD), mouse CD31 (CD31, Abcam, Inc, Cambridge, MA), or alpha smooth muscle actin (specific to human and mouse, α-SMA, Acris Antibodies, Inc, Rockville, MD), and biotinylated anti-rabbit Ig secondary Abs (Vector Laboratories, Burlingame, CA) were used for IHC staining.

Techniques: Staining, Microscopy, Software

Journal: Reproductive Sciences

Article Title: The Hyaluronic Acid System is Intact in Menstrual Endometrial Cells in Women With and Without Endometriosis

doi: 10.1177/1933719118766257

Figure Lengend Snippet: Western blot and IHC localization of CD44. Standard CD44 was expressed in both EECs and ESCs. This representative Western blot figure showed no difference; densitometric analysis showed no difference in protein from multiple samples among cells from women with and without endometriosis. IHC indicates immunohistochemistry; EECs, endometrial epithelial cells; ESCs, endometrial stromal cells, Endo, endometriosis; norm, normal.

Article Snippet: Rabbit-specific anti-HAS1 ( {"type":"entrez-nucleotide","attrs":{"text":"Ab198846","term_id":"57635958","term_text":"AB198846"}} Ab198846 ; Abcam Biotechnology, Inc, Cambridge, Massachusetts), anti-HAS2 (H-60; Santa Cruz Biotechnology, Inc, Dallas, Texas), anti-HAS3 (H-69; Santa Cruz Biotechnology, Inc), anti-HYAL1 (HPA002112; Sigma-Aldrich), anti-HYAL2 (Ab68608; Abcam Biotechnology, Inc), anti-CD44s (BS-4916R; Bioss Antibodies, Woburn, Massachusetts), HA-binding protein (HAbp; HABP2; Abcam Biotechnology, Inc), 11 and anti-actin (sc1616; Santa Cruz Biotechnology, Inc) were used.

Techniques: Western Blot, Immunohistochemistry

Journal: Frontiers in Veterinary Science

Article Title: PDGFD maintains ovine tail ADSCs in a proliferative state by suppressing CXCL8 and activating PI3K/MAPK signaling

doi: 10.3389/fvets.2026.1777426

Figure Lengend Snippet: Immunofluorescence identification of CD44 in ovine ADSCs. (a) CD44 immunofluorescence staining (red) shows strong positive expression localized to the cell membrane and cytoplasm. (b) DAPI staining (blue) marks the cell nuclei. (c) Merged image illustrates the subcellular localization of CD44.

Article Snippet: To reduce non-specific binding, cells were blocked with 1% bovine serum albumin (BSA, Bioss, Beijing, China) at room temperature for 30 min. After blocking, cells were incubated overnight at 4 °C with a primary antibody against CD44 (rabbit polyclonal antibody, Bioss, Cat No. bs-55039R, dilution 1:1,000).

Techniques: Immunofluorescence, Staining, Expressing, Membrane